微型PCR 反應芯片是生物芯片的一個重要發展方向，是實現快速分子生物學檢測的一個新興技術。在微型PCR 芯片中，變性、回火、延伸三個溫度區的精確控制直接影響到
生成產物的數量和特異性，因此至關重要。本文使用虛擬儀器技術構建溫度測控系統,將其應用于微型PCR 芯片的精確溫度控制,該系統精度高, 溫度波動較小。通過Labview 圖形化編程軟件和數據采集卡,大大縮短了系統組建時間，使溫度數據采集和顯示一次性完成。
關鍵字：聚合酶反應； Labview； 溫度控制


Abstract: Micro PCR chip is a new development of Biochips. By this novel method, precise
detection in molecular biology field can be realized rapidly. It is very important for micro PCR
chip to accurately control the temperature of melting, annealing and extension. It will influence
both the production and the specificity of result directly. In this paper, graphical development
software and DAQ data acquisition card has been used to realize high precise and less fluctuant temperature control on chip, which not only contribute to accomplish data display and acquisition simultaneously, but shorten time for system construction as well.
Key words:PCR,temperature control,Labview